KHẢO SÁT KHẢ NĂNG ỨNG DỤNG KỸ THUẬT NUÔI CẤY MÔ SẸO TẠO DỊCH HUYỀN PHÙ CÂY Drosera burmanni Vahl TRONG THU NHẬN HỢP CHẤT ANTHRAQUINONE

LINK DOWNLOAD MIỄN PHÍ TÀI LIỆU "KHẢO SÁT KHẢ NĂNG ỨNG DỤNG KỸ THUẬT NUÔI CẤY MÔ SẸO TẠO DỊCH HUYỀN PHÙ CÂY Drosera burmanni Vahl TRONG THU NHẬN HỢP CHẤT ANTHRAQUINONE": http://123doc.vn/document/554273-khao-sat-kha-nang-u-ng-dung-ky-thuat-nuoi-ca-y-mo-se-o-ta-o-di-ch-huye-n-phu-cay-drosera-burmanni-vahl-trong-thu-nha-n-hop-chat-anthraquinone.htm

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SUMMARY

The thesis         
Drosera burmanni  was carried out
at Plant Biotechnology lab, University of Natural Sciences; and Chromatography
room, Research Institute for Biotechnology and Enviromental Technology,
University of Agriculture and Forestry, HCM city from March to August, 2007.

Results:
- Callus of D. burmanni Vahl was cultured successfully by using
Gamb 
2,4-D (0,2 mg/l), succrose (20g/l), PVP (1,25 mg/l), pH was adjusted to
5,8 before autoclaving.
- Column Chromatography and Thin Layer Chromatography were used to
purify anthraquinone compound in D. burmanni. This compound was
used as authentic sample for HPLC technique.
- Roots of D. burmanni contained 3,01% dry weight (DW) of anthraquinone,
larger than 2,78% in leaves.
- Anthraquinone content of red-leaf D. burmanni Vahl took 0,78% of DW,
compared with 2,78% DW in green-leaf trees.
- Biomass of D. burmannis suspension contained 0,02% of fresh weight of
anthraquinone, while no quantifiable amounts of anthraquinone has been
found in spent medium.

Ho Chi Minh city, September, 2007.
Hoang Thi Thu


vi





 .i




 iii
 iv
Summary v


 vi






 ix






 x




 xii






 xiii

 1
1.1.  1
1.2.  2
1.3.  2
1.4.  2
 3
2.1. Drosera burmanni Vahl 3
2.1.1.  3
2.1.2.  3
2.1.3.  4
2.1.4.  6
2.1.5.  7
2.1.6.  8
2.1.7.  11
2.1.8.  12
2.1.8.1.  12
2.1.8.2.  12
2.1.8.3.  12
2.1.8.4.  14
2.1.8.5.  15
2.1.9.  16

h 17
2.2.1.  17
2.2.2.  18
2.2.2.1.   18
2.2.2.2.  19
2.2.3.  19
2.2.3.1.  19
2.2.3.2.  19


vii
2.2.3.3.  20
2.2.3.4.  20
2.2.3.5. 
 22
2.2.4. 
 22
2.2.4.1.  22
2.2.4.2.  22
2.2.4.3.  22
2.2.4.4. o 23
2.2.4.5.  23
2.2.4.6.  23
2.3.  24
2.3.1.   24
2.3.1.1.  24
2.3.1.2.  26
2.3.1.3.  27
2.3.2.  28
  31
 31
3.1.  31
3.1.1.  31
3.1.2.  33
3.1.2.1. 
 33
3.1.2.2. 
 34
3.1.2.3. 3: 

2,4-
 35
3.1.2.4. 
 36
3.1.2.5. 
 37
3.2. 







 D. burmanni 38
3.2.1.  38
3.2.2.  39

in vitro  40
 40
 40
 40
 41


viii
ong
 41

in vitro 43

 44

 45
 45
-  46
4.1.  46

 46

 48
4.1.3. 3: 

2,4-
 49

 54
4.1.5. 5: 
 56
4.2. 







 D. burmanni 58
4.3. 
 59
 59



 61

in vitro 62

 64

 66
  68
5.1.  68
5.1.1.   68
5.1.2.  68
5.2.  69

 70



ix












2,4-D : 2,4-dichlorophenoxy acetic acid
BAP : 6-benzylaminopurine
CRD : Completely Randomized Design ()

HPLC : High Performane Liquid Chromatography (





)
IAA : 3-indolyl-acetic acid
IBA : 3-indolebutyric acid
MS : Murashige & Skoog, 1962
NAA : naphthalene acetic acid
PVP : poly vinyl pyrrolidone
TCL : Thin Cell Layer (





)
TLC : Thin Layer Chromatography (





)


x




TRANG
Drosera burmanni  4
nh  Drosera burmanni Vahl 4
D. burmanni 5
nh 2.4. D. burmanni Vahl  7
 10
2.6. 



Drosera  15
 21
 21
2.9. 



 24
2.10. 





 26
 27
2.12. 













 29
D. burmanni in vitro 31
4.1. 















D.burmanni 


5 

(0,2 mg/l) 2,4-






i 52
4.2. 


















D. burmanni 

5 

 (0,2 mg/l) 2,4-D






 53
 55
  55
 55
4.4. 


 D. burmanni 57
pm 60
 60


xi
 60
 60


70 ppm 60
D. burmanni 
 61
D. burmanni 
 61
D. burmanni Vahl 63
4.13. 

D. burmanni Vahl 63
D. burmanni  65
D. burmanni 
 65
 
 66
4.17.  66


xii












TRANG



2.1. 

















 18
3.1. Quy t 39
3.2. 







 42
3.3. 









45
3.4. 









(

) 58








4.1
 46


 54



 59


xiii




TRANG
 Drosera 
7-methyljuglone 8
 Drosera 9

Drosera .13

v 

 33

 34
ng 3.3. 

2,4-D
 35

 36

 37

in vitro 43

c  44
  46

 47
 
 48
 
 48


xiv

 49

 54
 56
 59
 
in vitro 62
4.10. 










64
4.11. 







66