Xác định Gene liên quan đến tính kháng Virus PMWaV gây bệnh héo đỏ đầu lá trên cây dứa Cayenne bằng phương pháp PCR thoái hóa

v
MỤC LỤC



 iii
 iv
 v
 viii
 x
 xii
 xii
 1
 1
 3
 3
 3
 4
 4
 4
 4
Cayenne 5
 7
 8
 8
 9
 10
 10
 11
 11
 13

vi
 13
 14
 16
 18
- for - gene concept) 19
 20
 24
 24
 25
 27
 31
t 31
 32
 32
 32
 35
 36
riction Fragment Length Polymorphism) 37
 37
 37
 39
 39
 39
  39
 39
 39
 40
 41
 41

vii
  44
 49
 50
 50
 50
 51
4.1. 54
 56
 56
 56
 57
 64
4.2.5. PC 65
 66
 68
 69
 69
 69
 70



viii
DANH SÁCH CÁC CHỮ VIẾT TẮT

bp base pairs
cs. 
CC coiled coil
cDNA complementary deoxynucleic acid
CTAB cetyl trimethyl ammonium bromide
DNA deoxyribo nucleic acid
dNTP deoxyribonucleotide triphosphate
EB extraction buffer
EDTA ethylene diamine tetra acetic acid
ELISA enzyme-linked immunosorbent assay
EST expressed sequence tag
GLRaV-3 grape leafroll associated virus
KIN kinase
LRR leucine rich repeat
MAS marker assisted selection
MWP mealybug wilt of pineapple
NBS nucleotide binding site
OD optical density
PCR polymerase chain reaction
PCV pineapple closterovirus
PMWaV pineapple mealybug- wilt associated virus
QLT quatity loci trait
RFLP restriction fragment length polymorphism
RGA resistance gene analogs
RT reverse transcription
RT-PCR reverse transcription-polymerase chain reaction
TAE tris  acetate  EDTA
TBIA tissue blot immunoassay
TE tris  EDTA

ix
TIR toll interleukin receptor
TMV tobacco mosaic virus
Tp. HCM 

x
DANH SÁCH CÁC HÌNH

 5
 6
 8
9
 10
 11
 20
 22
c NBS-LRR. 23
 24

 27

 29
 34
 42
 43
 51
 52
 53
 53
 54
 55
 56
 57

 58

xi
 59
 60

2+
61
 62
 62
 63
 65
 66
 67
Hind III  68

xii
DANH SÁCH CÁC BẢNG
 22
 46
 . 47
 . 47
 49
 50
  55
 58



DANH SÁCH CÁC BIỂU ĐỒ
 45






1

Chƣơng 1
MỞ ĐẦU
1.1. Đặt vấn đề
           
 

   





          Pineapple mealybug wilt –
associated virus


 



         
  
 





2

 xem
 
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



 

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
 
         




   



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        
              





Xem chi tiết: Xác định Gene liên quan đến tính kháng Virus PMWaV gây bệnh héo đỏ đầu lá trên cây dứa Cayenne bằng phương pháp PCR thoái hóa